MicroSEQ™ ID Purification Combo Kit, with clean-up cartridges (Applied Biosystems™)

This MicroSEQ™ ID Purification Combo Kit includes reagents for post-PCR and post-sequencing steps in the MicroSEQ™ ID microbial identification workflow. It contains 2 tubes of ExoSAP-IT™ Reagent for PCR clean-up and 36 MicroSEQ™ ID Sequencing Clean-up Cartridges.

ExoSAP-IT™ Reagent: this reagent is used for quick post-PCR cleanup for downstream applications such as sequencing. It allows for removal of unconsumed dNTPs and primers remaining in the PCR mix following amplification.

ExoSAP-IT™ Reagent can be used downstream of the following PCR amplification kits:
• MicroSEQ™ 500 16S rDNA PCR Kit
• MicroSEQ™ Full Gene 16S rDNA PCR Kit
• MicroSEQ™ D2 rDNA Fungal PCR Kit

MicroSEQ™ ID Sequencing Clean-up Cartridges: these cartridges are used for post-sequencing clean-up in the MicroSEQ™ ID workflow. The gel filtration matrix used in the cartridges provide a convenient and simple way to remove dye terminators, dNTPs, and salts, as well as residual primers from cycle sequencing reactions.

The MicroSEQ™ ID Sequencing Cleanup Cartridges can be used downstream of the following sequencing kits:
• MicroSEQ™ 500 16S rDNA Sequencing Kit
• MicroSEQ™ Full Gene 16S rDNA Sequencing Kit
• MicroSEQ™ D2 rDNA Fungal Sequencing Kit