Pierce™ Nickel Coated Plates (Thermo Scientific™)

Key Features

Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These white plates can be used with chemiluminescent detection methods.

Features of Pierce Nickel Coated Plates include:
• Ni²⁺ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1 ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27 kDa) per well
• Activation level: 200 μL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

Order Codes

Code	Description
15442	Catalog Number: 15442 Unit Size: 5 plates Description: Clear, 96-well Detection Method: Colorimetric
15342	Catalog Number: 15342 Unit Size: 5 plates Description: Black, 96-well Detection Method: Fluorescence
15142	Catalog Number: 15142 Unit Size: 5 plates Description: Clear, 8-well Strip Detection Method: Colorimetric
15242	Catalog Number: 15242 Unit Size: 5 plates Description: White, 96-well Detection Method: Chemiluminescence