Bac-to-Bac™ HT Vector Kit (Gibco™)

The Bac-to-Bac™ HT Vector is designed for use as part of the Bac-to-Bac™ Baculovirus Expression System (Cat. No. 10359-016) for the expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli. The pFastBac™ HT vector offers the following features:

• Strong polyhedrin promoter for protein expression
• Three reading frames for simplified cloning
• N-terminal 6xHis tag for simple purification of recombinant fusion proteins
• TEV protease cleavage site for removal of the histidine tag following protein purification