BioNick™ DNA Labeling System (Invitrogen™)

The BioNick™ DNA Labeling System is ideal for generating biotinylated DNA probes by nick translation optimized for use in non-radioactive in situ hybridizations. These probes can also be used in Southern or northern blots, plaque lifts, colony hybridizations, and dot blot hybridizations. Using the BioNick™ DNA Labeling System:

• DNA is labeled with biotin-14-dATP, producing probe sizes from 50 to 500 bp
• One reaction labels 1 μg of template DNA