DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples (Invitrogen™)

DNAzol™ Reagent is a complete and ready-to-use organic reagent for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol™ Reagent procedure can be completed in typically 10–30 minutes with DNA recoveries of 70–100%. DNAzol™ Reagent is:

• Versatile—can use with a broad spectrum of sample materials
• Efficient—rapid isolation and high recovery of genomic DNA

Efficient isolation of genomic DNA from a variety of sample types
The DNAzol™ Reagent procedure uses a novel guanidine-detergent lysing solution that permits selective precipitation of DNA from cell lysate. 1 mL of DNAzol™ Reagent can be used to isolate genomic DNA from 1–3 x 10⁷ cells, from 0.1 mL of whole blood, or from 25–50 mg tissue.

Rapid isolation and high recovery of genomic DNA
The DNAzol™ Reagent combines reliability, efficiency, and simplicity in its DNA isolation protocol. During isolation, a biological sample is lysed (or homogenized) in DNAzol™ Reagent, and the genomic DNA is then precipitated from the lysate with ethanol. Following an ethanol wash, DNA may be solubilized in either water or 8 mM NaOH. The entire procedure can be completed in 10–30 min with DNA recovery of 70–100%.

Isolated DNA can be used for a number of downstream applications
Genomic DNA extracted using the DNAzol™ procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization.