GeneChip™ WT Terminal Labeling and Controls Kit (Applied Biosystems™)

The GeneChip™ WT Terminal Labeling and Controls Kit should be combined with the WT Expression Kit (sold separately) as the most effective option for target preparation on GeneChip Exon and Gene 1.0 ST Arrays. The basic chemistry is the same as the GeneChip WT Sense Target Labeling and Control Reagents kit; however, the WT Expression Kit utilizes a streamlined protocol with master mixes, does not require an upfront rRNA-reduction step for optimal performance, and uses optimized reagents that enable lower total RNA input amounts.

The GeneChip WT Terminal Labeling and Controls Kit contains the GeneChip WT Terminal Labeling Kit, GeneChip Poly-A Control Kit, and GeneChip Hybridization Control Kit.

Key Features

WT Expression Kit:
   - 50 ng total RNA input
   - No ribosomal removal required for Human, Mouse, and Rat Exon 1.0 ST and Gene 1.0 ST Arrays
   - Streamlined workflow for a simplified protocol and reduced hands-on time
   - Magnetic bead-based purification for enhanced recovery

The WT Expression Kit includes:
   - All reagents needed to generate sense cDNA ready for fragmentation and labeling
   - Magnetic beads for enhanced purification efficiency
   - Plastic consumables required to run the assay

The GeneChip WT Terminal Labeling and Controls Kit includes:
   - WT Fragmentation and Labeling Kit required for target produced by the WT Expression Kit
   - Poly-A controls that are added to input RNA to measure efficiency of target amplification by the WT Expression Kit
   - Hybridization controls that monitor the hybridization process for troubleshooting purposes

What you need to order
• For 10 reactions:
   - WT Expression Kit (Cat. No. 4411973), 10 reactions
   - GeneChip WT Terminal Labeling and Controls Kit (Cat. No. 901525), 10 reactions*
   *Additional GeneChip WT Terminal Labeling Kit, 10 reactions (Cat. No. 900670), is available separately.

• For 30 reactions:
   - WT Expression Kit (Cat. No. 4411974), 10 reactions
   - GeneChip WT Terminal Labeling and Controls Kit (Cat. No. 901524), 10 reactions*

Documentation
The following documentation is required for using the WT Expression Kit and the GeneChip WT Terminal Labeling and Controls Kit with Exon and Gene 1.0 ST Arrays.
• Protocol, WT Expression Kit (Cat. No. 4425209)
• User Manual, GeneChip WT Terminal Labeling and Hybridization (Cat. No. 702808)
• User Manual, GeneChip Expression Wash, Stain and Scan (Cat. No. 702731)

Related Links
GeneChip™ Hybridization, Wash, and Stain Kit
GeneChip™ WT Terminal Labeling and Controls Kit
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