pcDNA™3.3-TOPO™ TA Cloning™ Kit (Invitrogen™)

The pcDNA™3.3-TOPO™ TA Cloning™ Kit is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.3™-TOPO™ TA Cloning™ Kit is supplied with the TOPO™-adapted plasmid vector, all reagents for cloning, and One Shot™ TOP10 competent cells. The pcDNA™3.3-TOPO™ vector offers the following benefits:

• TOPO™ adapted for cloning PCR products with >85% efficiency
• High-level gene expression in a wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promoter/enhancer sequence (672 bp)
• Vector is ideal for large-scale protein expression
• Generation of native protein without extraneous amino acids

The pcDNA™3.3-TOPO™ vector is also included with the OptiCHO™ Antibody Express System for cloning and expression of light and heavy antibody chains.