PureLink™ HiPure Expi Plasmid Gigaprep Kit (Invitrogen™)

The PureLink™ HiPure Expi Plasmid Gigaprep Kit enables isolation of the highest levels of transfection-grade plasmid DNA from a single E.coli giga prep using an enhanced anion exchange resin. The PureLink™ HiPure Plasmid Gigaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times. The PureLink™ HiPure Expi Plasmid Gigaprep Kit typically isolates 12 mg of high quality, ultrapure plasmid DNA with inherently low endotoxin levels from 2.5 L of bacterial culture.

Advantages of the PureLink™ HiPure Expi Plasmid Gigaprep Kit include:
• High yield—isolate up to 14 mg of high quality plasmid DNA from a single purification using 5 L of bacterial culture volume
• Purity—inherently low endotoxin levels (typically 0.1–1.0 EU/μg) and A260/280 >1.8, making it ideal for mammalian cell transfection
• Simple and super-fast protocol—with vacuum assisted protocol, typical plasmid isolation in as little as 90 minutes

HiPure Expi anion exchange chromatography for plasmid purification
PureLink™ HiPure Expi plasmid purification kits use a novel, proprietary anion-exchange resin to enable the purification of the highest levels of transfection-grade plasmid DNA. The enhanced resin combines large capacity with fast flow rate, high resolution, and efficient endotoxin removal. Plasmid preparations are typically completed in less than 2 hours with purity equal to two CsCl gradients. PureLink™ HiPure Expi plasmid purification kits enable the quickest process for obtaining the highest amount of transfection-grade plasmid DNA.