Quant-iT™ microRNA Assay Kit (Invitrogen™)

Quantitate microRNA easily and accurately with the Quant-iT microRNA Assay Kit, a microRNA (miRNA) detection kit that detects miRNA even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for microRNA over rRNA or large mRNA (>1000 bp).

Although not exclusively selective for miRNA, the assay reagent can reproducibly quantify miRNA in pure samples down to levels as low as 0.5 ng following the supplied protocol, even in the presence of mRNA.

Features of the Quant-iT microRNA Assay Kit include:
• Sensitive detection of as little as 0.5 ng miRNA in a microplate well.
• A core dynamic range of 5–500 ng/mL.
• Accurate readings of sample concentrations ranging from 50 ng/mL to 100 μg/mL.

The Quant-iT microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent 1:200, load 200 μL into the wells of a microplate, add 1–20 μL of sample, mix, and read the fluorescence. The assay is performed at room temperature, and the signal is stable for approximately three hours. Because the assay tolerates 1–20 μL of sample in an assay volume of 200 μL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100 μg/mL.