RNAqueous™ Total RNA Isolation Kit (Invitrogen™)

The RNAqueous™ Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 10²–10⁷ cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous™ Kit provides:

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous™ Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.