AgriSeq™ Canine SNP Parentage and ID Panel (Applied Biosystems™)

The Applied Biosystems AgriSeq Canine SNP Parentage and Identification Panel contains 381 SNP markers for parentage determination and identification of canines by targeted genotyping-by-sequencing (GBS). The panel enables multiplexed simultaneous genotyping using the AgriSeq targeted GBS workflow. The CanFam 3.1 reference genome is used for the analysis.

The need for highly consistent detection of informative genetic markers is critical for genetic trait detection. Targeted GBS methods, like AgriSeq, have the advantage over non-targeted GBS approaches (e.g., RADSeq) that are highly susceptible to allele drop-outs and missing data. Using the AgriSeq workflow, hundreds to thousands of markers can be targeted simultaneously in a highly reproducible manner across diverse sample sets. In collaboration with multiple experts and ISAG member laboratories, we have developed and validated the performance of all 381 SNP markers in the panel. The performance of the panel was verified using orthogonal testing, robustness testing, and field testing with diverse canine oral swab DNA samples. The panel was also qualified using the 2019 ISAG/ICAR 2nd SNP Typing Canine Comparison samples. The comprehensive AgriSeq Canine SNP Parentage and Identification Panel is designed to deliver reliable genotyping results for parentage determination and identity verification.

Features of the AgriSeq Canine SNP Parentage and Identification Panel include:
• Reproducible genotyping of 381 SNP markers for parentage and identity determination
• Simple, high-throughput workflow
• Customizable approach to combine with compatible traits and disorders markers

>99.5% mean sample call rate for markers
A high genotype call rate and reproducibility are imperative to accurately determine parentage and identity. Highly diverse canine oral swab DNA field samples were evaluated using the AgriSeq targeted GBS workflow, and a 98.5% mean sample call rate was obtained for majority of the markers (see figure below). Replicate genotype concordance was >99.9% and calls were highly concordant with orthogonal data (>99%).

Simple, high throughput workflow
The AgriSeq Canine SNP Parentage and Identification Panel has been optimized for use in the AgriSeq targeted GBS workflow. Up to 768 unique samples can be pooled per chip, greatly reducing the sequencing cost and labor per animal. Up to four 384-well plates can be processed in a single day and full sequencing results can be obtained in as little as three days. The flexibility of the AgriSeq workflow allows hundreds of samples to be pooled together into a single sequencing run targeting hundreds to thousands of markers.

Combine with AgriSeq Canine Traits and Disorders Panel or customize panel with additional markers for maximal test value
The AgriSeq targeted GBS workflow is flexible enough to add compatible markers and panels together for efficient, high-value genotyping. Whether you are looking to combine parentage analysis with specific desired trait markers or genetic defect markers to cull out carriers in breeding programs, our dedicated team of agrigenomics bioinformatics specialists is available to help design high performance panels.

The AgriSeq Canine SNP Parentage and Identification panel, along with the AgriSeq workflow, provides a streamlined, cost-effective method for canine parentage verification and identity determination.

NOTE: The CanFam 3.1 reference genome needed for analysis of the AgriSeq Canine SNP Parentage and Identification Panel can be obtained by contacting technical support.