ChargeSwitch™-Pro PCR Clean-Up Kit (Invitrogen™)

The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.

The ChargeSwitch™-Pro Kit provides:
• 100% ethanol-free, guanidinium-free, organic solvent-free protocols
• Improved downstream performance
• Complete kit for fast, hassle-free setup
• 20% fewer handling steps than most PCR clean-up protocols
• Purification of up to 500 μL of PCR product in one column


How It Works
ChargeSwitch™ technology employs a completely novel mechanism that replaces harsh reagents such as chaotropic salts, ethanol, or organic solvents and isopropanol precipitation with a simple ion-exchange procedure. In this method, DNA binds to a surface ligand that is positively charged at low pH, and is eluted when the pH is raised to 8.5 (Figure 1). The ChargeSwitch™-Pro protocol retains the ease and familiarity of common silica spin-column protocols with binding, washing, and elution steps (Figure 2).


Better Downstream Results
Since ChargeSwitch™ technology does not require the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification, the resulting DNA is free of enzymatic inhibitors and thus shows better downstream performance (Figure 3). These compounds are known to inhibit many enzymatic reactions such as PCR, cloning, restriction enzyme digestion, and array CGH labeling. Expect to see reduced variability in your sequencing applications, with reads in excess of 800 bp with Phred scores >20 (Figure 4); clean restriction enzyme digestions (Figure 5); and removal of dNTPs and primers in excess of 98% (Figure 6).