DNA Polymerase I/DNase I (Invitrogen™)

DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA.

Application:
Labeling DNA with either radiolabeled or biotinylated nucleotides.

Source:
DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964.

Performance and Quality Testing:
Performance tested in nick translation reaction.

Unit Definition:
One unit of DNA Polymerase I in the absence of DNase I incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.

Unit Reaction Conditions:
50 mM potassium phosphate (pH 7.5), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 μg/ml template•primer, 32 μM dTTP, 69 nM [3H]dTTP, and enzyme in 100 μl for 30 min. at 37°C.

Order Codes

Code Description
18162016 Catalog Number: 18162016
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