High Resolution Melt (HRM) App (Applied Biosystems™)

The High Resolution Melt (HRM) App hosted on Thermo Fisher Cloud is a tool for HRM analysis, which is a post-PCR analysis method used to identify variation in nucleic acid sequences. The method is based on detecting small differences in PCR melting (dissociation) curves. It is enabled by high-brightness, dsDNA-binding dyes used in conjunction with real-time PCR instrumentation that has precise temperature ramp control, advanced data capture capabilities, and access to software designed specifically for HRM analysis.

Key product features:
• Minimal user input—app automatically applies default/saved analysis settings upon opening an experiment file
• High accuracy—clustering algorithm accurately distinguishes control and variant genotypes
• High sensitivity—app allows input of expected number of clusters for difficult-to-detect SNPs (class 4)
• Efficiency—analyze multiple runs simultaneously and visualize the data

Minimize user input and easily recall analysis settings
The HRM App automatically applies default or saved analysis settings upon opening an experiment file, which allows you to achieve results with minimal input.

Realize high accuracy and sensitivity
The HRM App uses the same clustering algorithm as the desktop version, which helps to accurately distinguish control and variant genotypes, and allows the user to input the expected number of clusters for increased sensitivity for difficult SNP genotyping. It also offers data compatibility with the desktop version.

Achieve more with less work
With the HRM App, you can analyze multiple plates simultaneously. Unlike the desktop version, you can access an additional set of data review tools, such as those for amplification plots and multicomponent data.

App subscription
The following subscription plans are available at rates for 1 user or 10 users. (Please contact us if our available plans do not meet your needs.) A free trial is available for first-time users. The Terms and Conditions for Software Application Subscription Plans on the Thermo Fisher Cloud apply to each subscription plan described below:

• Auto-renewal Monthly:This plan is charged month-to-month. Each month is paid up front and the subscription auto-renews every month. This plans provides the most flexibility with the lowest up-front costs.
• Auto-renewal Annual: This plan is charged once a year. Each year is paid up front and auto-renews every year. This plan is more cost-effective than the month-to-month plan.
• Termed Contract for One Year or Five Years: With this plan the entire term of the contract is paid up front and does not renew. This plan is most suitable when a capital expenditure is more desirable than smaller recurring payments.