MultiScribe™ Reverse Transcriptase (Invitrogen™)

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays. MultiScribe Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. This recombinant RNA–dependent, DNA polymerase uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand.

Features of MultiScribe Reverse Transcriptase include:
• Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay setup
• Individual component design provides versatility in assay setup
• 5,000 U is supplied in 100 μL vials (50 U/μL)
• Optimal reaction temperature: 42–52°C

Applications
• PCR
• RT-PCR
• Reverse Transcription