PathoProof™ Majorû3 Kits for Applied Biosystems™ 7500 and 7500 Fast qPCR systems (Thermo Scientific™)

Accurately and rapidly identify three of the main mastitis-causing microorganisms using Thermo Scientific™ PathoProof™ Major-3 Kits for the Applied Biosystems™ 7500 and 7500 Fast qPCR systems. Obtain results within four hours and identify the particular pathogen causing the infection by testing either fresh or preserved milk samples. Make faster decisions on treatment and reduce the time that an infective agent has to spread in a herd.

Key Features

Good-quality raw milk must meet accepted standards for chemical composition and purity as well as levels of a variety of mastitis-causing microorganisms. Identify three of the main mastitis-causing microorganisms and the Staphylococcal β-lactamase gene responsible for the penicillin resistance in Staphylococci using PathoProof Major–3 multiplex PCR. All targeted species are detected simultaneously, overcoming the common problem of mixed growth results in bacterial culturing.

Ensure milk and milk products are safe and suitable for their intended uses
Remove affected cows from the herd to ensure optimal milk quality
Enables faster decisions on treatment
Reduces time available for infective agents to spread to healthy herd mates
Reduces broad spectrum antibiotic treatment
Rapid treatment shortens inflammation time

Assays are based on real-time PCR, an accurate and sensitive technology for identification of the target DNA in the sample. Tests can be used for analyzing fresh and preserved quarter milk samples, milk recording samples, and bulk tank milk samples.

Achieve high sensitivity, even for samples that show “no growth” in culture. Accurate detection of viable, dead, and growth-inhibited microorganisms reduces “no-growth” results.

PathoProof Major-3 Kit identifies:

1. Mycoplasma bovis

2. Staphylococcus aureus

3. Streptococcus agalactiae

Automated sample preparation

Significantly reduce the hands-on time needed when processing larger numbers of samples with the semi-automated Thermo Scientific™ KingFisher™ Flex instrument for magnetic bead-based DNA purification and extraction, along with PathoProof Major-3 KingFisher Kits and qPCR reagents.

Not all products are available for sale in all territories. Please inquire.

Order Codes

Code	Description
PF3050SB	Catalog Number: PF3050SB Unit Size: Each Description: qPCR reagents for use with PF3050S For Use With (Equipment): For use with Applied Biosystems 7500 qPCR systems. Must be ordered together with PF3050S. No. of Tests: 50 Tests
PF3050S	Catalog Number: PF3050S Unit Size: Each Description: PathoProof Major-3 kit For Use With (Equipment): Must be ordered together with PF3050SB qPCR Reagents. No. of Tests: 50 Tests
PF3050L	Catalog Number: PF3050L Unit Size: Each Description: PathoProof Major-3 kit For Use With (Equipment): Must be ordered together with PF3050LB qPCR Reagents. No. of Tests: 384 Tests
PF3050LB	Catalog Number: PF3050LB Unit Size: Each Description: qPCR reagents for use with PF3050L and PF3050LKF For Use With (Equipment): For use with Applied Biosystems 7500 qPCR systems. Must be ordered together with PF3050L or PF3050LKF. No. of Tests: 384 Tests