PCR Master Mix (2X) (Thermo Scientific™)

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

• Convenient, ready-to-use mix
• Thermostable—half life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling

Note

• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10^-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 10⁴. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.