Platinum™ GenoType Tsp DNA Polymerase (Invitrogen™)

Platinum™ GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic 'hot-start' method that improves PCR specificity and allows for room temperature reaction assembly.

Platinum™ GenoType Tsp DNA Polymerase:
• Exhibits minimal non-templated nucleotide addition to PCR products
• Lacks both 5´ and 3´ exonuclease activity
• Amplifies fragments up to 500 bp

Application
Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.

Unit definition
One unit of Platinum™ GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C under optimized reaction conditions.