RapidFinder™ Turkey ID Kit (Thermo Scientific™)

The RapidFinder™ Turkey ID Kit enables the simple and reliable detection of turkey in food and feed samples. The method works by detection of turkey, or Meleagris gallopavo, DNA by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Turkey ID Kit detects both raw and processed turkey and provides sensitivity down to 0.01% turkey DNA. The included positive control in the kit, which comprises 0.1% turkey DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-turkey DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Turkey ID Kit Works
Turkey DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for mitochondrial DNA sequence specific to Meleagris gallopavo. The target sequence, detected in the FAM™ dye channel, is not only specific for turkey, but also stable. Also included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% turkey DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems™ StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Turkey ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Turkey ID Kit, most labs will go on to quantify the amount of turkey DNA in the sample. This can be accomplished using the RapidFinder™ Turkey ID Kit in conjunction with the RapidFinder™ Quant Multi-Meat Set (Cat. No. A24399).

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Turkey ID Kit is just one of our many available kits for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of pork, beef, horse, sheep, ruminant, chicken, and poultry.

The RapidFinder™ Meat Species Identification Kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).