TrueMark™ Lesion Plus Combo Kit (Applied Biosystems™)

The Applied Biosystems TrueMark Lesion Plus Combo Kit achieves molecular detection through real-time PCR technology, offering an end-to-end workflow for many common infectious disease areas by utilizing the power of QuantStudio real-time PCR systems. The combination of a TrueMark panel (included) and a QuantStudio system helps enable rapid, accurate detection and categorization of microorganisms that cause lesion diseases.

The TrueMark Lesion Plus panel is composed of convenient, duplexed TaqMan assays that detect herpes simplex virus type 1 (HSV1), herpes simplex virus type 2 (HSV2), cytomegalovirus, Haemophilus ducreyi, Treponema pallidum, varicella-zoster virus, Lymphogranuloma Venereum, Pan-Candida, and an internal control in the same reaction, utilizing real-time PCR techniques.

Features of the TrueMark Lesion Plus panel include:
• Ease-of-use—convenient, duplexed reactions include assay and positive control, pre-spotted and dried down on TaqMan plates, designed for easy setup and increased accuracy; just add sample and TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX) (included)
• Endogenous controls—duplex reactions offer RNase P human internal control in every well for added confidence
• Turn-around time—choose from a selection of predefined panels with a short turn-around time
• Amplification control—TrueMark Amplification Control I covers all targets in RTI, UTI, STI, and vaginal panels
• Maximize the workflow—expand from SARS-CoV-2 testing to TrueMark infectious disease research panels utilizing the same workflow

The TrueMark Lesion Plus Combo Kit includes the following components:
• TrueMark Lesion Plus Panel—collection of duplex assays that contain primer/probe sets specific to herpes simplex virus type 1 (HSV1), herpes simplex virus type 2 (HSV2), cytomegalovirus, Haemophilus ducreyi, Treponema pallidum, varicella-zoster virus, Lymphogranuloma Venereum, Pan-Candida, and RNase P genomic regions
• TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX)—ready-to-use PCR mix, including reverse transcriptase, polymerase, dNTPs, salt, and buffer

Product use
The TrueMark Lesion Plus Panel is a collection of duplex assays that detect microbes and an internal control in the same reaction for qualitative detection and identification of herpes simplex virus type 1 (HSV1), herpes simplex virus type 2 (HSV2), cytomegalovirus, Haemophilus ducreyi, Treponema pallidum, varicella-zoster virus, Lymphogranuloma Venereum, Pan-Candida in lesion/genital swab obtained from individuals exhibiting signs and symptoms of genital ulcer.