TrueMark™ Respiratory I Plus Combo Kit (Applied Biosystems™)

The Applied Biosystems TrueMark Respiratory I Plus Combo Kit achieves molecular detection through real-time PCR technology, offering an end-to-end workflow for many common infectious disease areas by utilizing the power of QuantStudio real-time PCR systems. The combination of a TrueMark panel (included) and a QuantStudio system helps enable rapid, accurate detection and categorization of microorganisms that cause respiratory diseases.

The TrueMark Respiratory I Plus panel is composed of convenient, duplexed TaqMan assays that detect adenovirus, enterovirus, influenza A (Pan), influenza B, respiratory syncytial virus A/B, rhinovirus, human metapneumovirus, SARS-CoV-2, and an internal control in the same reaction, utilizing real-time PCR techniques.

Features of the TrueMark Respiratory I Plus panel include:
• Ease-of-use—convenient, duplexed reactions include assay and positive control, pre-spotted and dried down on TaqMan plates, designed for easy setup and increased accuracy; just add sample and TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX) (included)
• Endogenous controls—duplex reactions offer RNase P human internal control in every well for added confidence
• Turn-around time—choose from a selection of predefined panels with a short turn-around time
• Amplification control—TrueMark Amplification Control I covers all targets in RTI, UTI, STI, and vaginal panels
• Maximize the workflow—expand from SARS-CoV-2 testing to TrueMark infectious disease research panels utilizing the same workflow

The TrueMark Respiratory I Plus Combo Kit includes the following components:
• TrueMark Respiratory I Plus Panel—collection of duplex assays that contain primer/probe sets specific to adenovirus, enterovirus, influenza A (Pan), influenza B, respiratory syncytial virus A/B, rhinovirus, human metapneumovirus, SARS-CoV-2, and RNase P genomic regions
• TrueMark Infectious Disease Panels 1-Step Multiplex Master Mix (No ROX)—ready-to-use PCR mix, including reverse transcriptase, polymerase, dNTPs, salt, and buffer

The TrueMark Respiratory I Plus Combo Kit is used with nasopharyngeal swab or nasopharyngeal aspirate samples on our QuantStudio real-time PCR systems.

Product use
The TrueMark Respiratory I Plus Panel is a collection of duplex assays that detect microbes and an internal control in the same reaction for qualitative detection and identification of adenovirus, enterovirus, influenza A (Pan), influenza B, respiratory syncytial virus A/B (RSV A/B), rhinovirus, human metapneumovirus (HM), SARS-CoV-2 in nasopharyngeal (NP) or nasopharyngeal aspirate swabs obtained from individuals exhibiting signs and symptoms of a respiratory tract infection.