AccelerOme™ TMT11plex MS Sample Preparation Kits (Thermo Scientific™)

Key Features

Features of the TMT11plex MS Sample Preparation Kit include:
• Optimized—EasyPep kit chemistry combined with TMT11plex labeling reagents specifically designed for use with the AccelerOme Automated Sample Prep System
• Convenient—complete set of ready-to-use proteomic sample prep, clean-up, and TMT-labeling reagents for easy loading onto the instrument
• Robust—reagents combined with AccelerOme ecosystem provide reproducible, high-quality TMT-labeled peptides with greater than 95% labeling efficiency
• Flexible—sample prep and TMT-labeling reagents verified on system using cells, plasma, proteins, and tissue samples for 10–100 μg of sample input
• Multiplex—enables concurrent mass spectrometry analysis of up to 11 samples

The AccelerOme TMT11plex MS Sample Preparation Kit integrates pre-formulated buffers, MS-grade trypsin/Lys-C enzyme mix, and peptide clean-up cartridges with TMT11plex label reagents for reproducible sample preparation of TMT-labeled peptides. These single-use components are conveniently provided in labeled trays for direct placement on the AccelerOme system. Additionally, a peptide retention time calibration standard is also included with the kit that can be spiked into the sample before LC-MS analysis to assess system performance. This kit provides a complete reagent solution for automated sample preparation of TMT11plex labeled samples to produce high quality, mass spectrometry ready peptides.

The AccelerOme reagent portfolio is an integral part of the AccelerOme Automated system solution to provide robust proteomic mass spec sample preparation. The instrument hardware is built on a proven liquid handling format for reproducible sample processing and optimized on-line μSPE sample clean-up for excellent peptide recovery. TMT label reconstitution and sample labeling and pooling have been incorporated before sample clean-up. Peptide quantitation post-clean-up is achieved through accurate UV spectrophotometric analysis to determine peptide concentrations prior to LC-MS analysis. The complete system (kits + reagents + software) was designed to provide high quality peptides while improving productivity with an easy-to-use automated system. When combined with the industry-leading, high-resolution Orbitrap instruments and software, TMT11plex reagents provide integrated total solutions for quantitative protein expression analysis by mass spectrometry.

These products are subject to a limited use label license.

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