BioPrime™ Array CGH Genomic Labeling Module (Invitrogen™)

The BioPrime™ Plus Array CGH Genomic Labeling Systems are a high-performance labeling kits that enable reproducible labeling of genomic DNA samples for array-based Comparative Genomic Hybridization (CGH). Available in both indirect and direct labeling formats, the BioPrime™ Plus Array CGH Genomic Labeling Systems provide a flexible solution to your genomic labeling needs.

Using the BioPrime™ Plus Array CGH Genomic Labeling Systems, you can expect:

• High yields of fluorescently labeled genomic DNA
• signal-to-noise ratios
• detection of gene copy number variations (Figure 1)

These systems combine a highly concentrated exo-Klenow fragment of DNA polymerase I and random primers to effectively label genomic targets for sensitive genomic profiling experiments. Exo-Klenow is a mutant of the large fragment of the DNA polymerase I holoenzyme that has both 5' - 3' and 3' - 5' exonuclease activity removed. The lack of exonuclease activity makes this enzyme ideal for use in random priming protocols, enabling robust yields and efficient incorporation of Alexa Fluor™ AHA fluorescent nucleotides. In the BioPrime™ Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor™ AHA modified nucleotides (direct labeling) or amino-allyl modified nucleotides (indirect labeling) are incorporated as the polymerase extends from the priming sites. The labeled samples are then purified to remove contaminants prior to denaturing and hybridization to a microarray (direct labeling systems) or coupled to the Alexa Fluor™ NHS ester (indirect labeling systems) prior to hybridization (Figure 2).