CaptureSelect™ CH1-XL RoboColumn (Thermo Scientific™)

CaptureSelect RoboColumn™ small chromatography columns are used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, thereby enabling purification of all Fab fragments, independent of the light-chain type (kappa or lambda) and without binding of over-expressed light chain and light-chain dimers. In addition, it can be used for the purification of human IgGs from all four subclasses (1, 2, 3, and 4).

Features of this affinity matrix include:
• Platform approach for purification of high quality and correctly assembled human Fab fragments
• No co-purification of over-expressed free light chains due to selectivity for the CH1 domain
• Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
• Caustic cleaning with up to 50 mM NaOH
• Excellent scalability
• No cross-binding to bovine IgG, therefore presence of fetal bovine serum is not a problem
• Non-animal-derived

Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

Column size: 200 μL and 600 μL, 1 strip, 8 columns (8-row)
Column dimension: 200 uL: 5 mm D x 10 mm L
Column dimension: 600 uL: 5 mm D x 30 mm L
Matrix: agarose based, epoxide-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect CH1-XL affinity ligand
Ligand coupling: epoxide coupling
Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs, lower capacities may be observed.
Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5
Formulation buffer: 20% (v/v) ethanol
Operating pressure: 2 bar (maximum)
Flow rate: 16–150 cm/h

RoboColumn is a trademark of Repligen.