CaptureSelect™ Human Growth Hormone Affinity Matrix (Thermo Scientific™)

CaptureSelect Human Growth Hormone (hGH) Affinity Matrix has been specifically designed for the purification of human growth hormone from recombinant sources.

Features of this affinity matrix include:
• Highly selective for recombinant hGH
• Efficient purification of recombinant hGH with high yields and retained activity
• Excellent scalability
• Non-animal-derived

Recombinant hGH, also known as somatotropin or somatropin, is a peptide hormone that stimulates growth, cell reproduction, and regeneration in humans and other animals. The high selectivity and yields obtained using CaptureSelect Human Growth Hormone Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step.

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Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect hGH affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ∼3 mg/mL resin
Elution conditions: 20 mM citric acid, pH 3.0
Flow characteristics: 50–200 cm/h (up to 2 bar)
Formulation buffer: 20%(v/v) ethanol