CaptureSelect™ IgE Affinity Matrix (Thermo Scientific™)

Thermo Scientific CaptureSelect IgE Affinity Matrix has been designed specifically for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources.

Features of this affinity matrix include:
• Purification of human IgE, binding different recombinant IgE forms
• Efficient removal of host cell proteins (HCP) and IgE aggregates
• Excellent scalability
• Non–animal-derived

Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 μm
Ligand: CaptureSelect IgE affinity ligand
Ligand coupling method: aldehyde coupling
Binding capacity: >5 mg/mL resin
Elution conditions: 50 mM sodium citrate, 150 mM NaCl, pH 3.5
Flow characteristics: 50–200 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol