Click-IT™ ManNAz Metabolic Glycoprotein Labeling Reagent (tetraacetylated N-Azidoacetyl-D-Mannosamine) (Invitrogen™)

The Click-iT™ ManNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify and characterize cell surface sialic acid-containing glycoproteins. In step one, cultured cells are incubated with the azide-modified mannosamine (ManNAz). The azido-sugar is metabolically incorporated into cell surface sialic acid-containing glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway,. In step two, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT™ Glycoprotein Detection kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne). These Click-iT™ products are compatible with LC-MS⁄MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.