Click-iT™ sDIBO Alkynes for copper-free click chemistry (Invitrogen™)

Achieve efficient labeling of azides with Click-It sDIBO Alkynes. Our Click-IT sDIBO alkynes enable the labeling of azido labeled targets without the need for copper catalysis and are available with a variety of Alexa Fluor or biotin labels, as well as amine or thiol reactive groups for creating your own conjugates. Macromolecules that have been azido-modified enzymatically, chemically, or metabolically can now be labeled easily, yielding soluble bioconjugates.

Key Features

Click-iT sDIBO Alkyne labels react with azides via a copper-free Click chemistry reaction to form a stable triazole linkage with an Alexa Fluor, Biotin, or label of your choice. Copper-free click reactions using our sDIBO alkynes are ideal for surface labeling of live cells; they also minimize damage to fluorescent proteins like GFP or R-PE. For intracellular labeling, the nonspecific binding of DIBO alkynes creates a background signal, and a labeling strategy using picolyl azides is the preferred solution in these instances.

Click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be labeled more easily, yielding more soluble bioconjugates with improved biological labeling utility.

To make a concentrated stock solution from 2–5 mM, dissolve the dye-alkyne in high quality anhydrous DMSO. Store frozen, desiccated, and protected from light. Click-iT SDP Ester sDIBO alkyne is susceptible to hydrolysis, so prepare the stock solution fresh on the day of the experiment.

Order Codes

Code Description
C10407 Catalog Number: C10407
Unit Size: 500 µg
Label or Dye: Alexa Fluor™ 594
Reactive Moiety: DIBO Alkyne
Molecular Weight (g/mol): ∼1200
C20020 Catalog Number: C20020
Unit Size: 0.5 mg
Label or Dye: Alexa Fluor™ 488
Reactive Moiety: sDIBO Alkyne
Molecular Weight (g/mol): ∼1400
C20021 Catalog Number: C20021
Unit Size: 0.5 mg
Label or Dye: Alexa Fluor™ 555
Reactive Moiety: sDIBO Alkyne
Molecular Weight (g/mol): ∼1800
C20022 Catalog Number: C20022
Unit Size: 0.5 mg
Label or Dye: Alexa Fluor™ 647
Reactive Moiety: sDIBO Alkyne
Molecular Weight (g/mol): ∼1900
C20023 Catalog Number: C20023
Unit Size: 1 mg
Label or Dye: Biotin
Reactive Moiety: sDIBO Alkyne
Molecular Weight (g/mol): ∼1000
C20025 Catalog Number: C20025
Unit Size: 1 mg
Label or Dye: SDP ester
Reactive Moiety: SDP ester, sDIBO Alkyne
Molecular Weight (g/mol): ∼1050
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