Achieve efficient labeling of azides with Click-It sDIBO Alkynes. Our Click-IT sDIBO alkynes enable the labeling of azido labeled targets without the need for copper catalysis and are available with a variety of Alexa Fluor or biotin labels, as well as amine or thiol reactive groups for creating your own conjugates. Macromolecules that have been azido-modified enzymatically, chemically, or metabolically can now be labeled easily, yielding soluble bioconjugates.
Click-iT sDIBO Alkyne labels react with azides via a copper-free Click chemistry reaction to form a stable triazole linkage with an Alexa Fluor, Biotin, or label of your choice. Copper-free click reactions using our sDIBO alkynes are ideal for surface labeling of live cells; they also minimize damage to fluorescent proteins like GFP or R-PE. For intracellular labeling, the nonspecific binding of DIBO alkynes creates a background signal, and a labeling strategy using picolyl azides is the preferred solution in these instances.
Click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background. Macromolecules that have been azide-modified enzymatically, chemically, or metabolically can be labeled more easily, yielding more soluble bioconjugates with improved biological labeling utility.
To make a concentrated stock solution from 2–5 mM, dissolve the dye-alkyne in high quality anhydrous DMSO. Store frozen, desiccated, and protected from light. Click-iT SDP Ester sDIBO alkyne is susceptible to hydrolysis, so prepare the stock solution fresh on the day of the experiment.
| Code | Description |
|---|---|
| C20021 | Catalog Number: C20021 Unit Size: 0.5 mg Label or Dye: Alexa Fluor™ 555 Reactive Moiety: sDIBO Alkyne Molecular Weight (g/mol): ∼1800 |
| C10407 | Catalog Number: C10407 Unit Size: 500 µg Label or Dye: Alexa Fluor™ 594 Reactive Moiety: DIBO Alkyne Molecular Weight (g/mol): ∼1200 |
| C20020 | Catalog Number: C20020 Unit Size: 0.5 mg Label or Dye: Alexa Fluor™ 488 Reactive Moiety: sDIBO Alkyne Molecular Weight (g/mol): ∼1400 |
| C20022 | Catalog Number: C20022 Unit Size: 0.5 mg Label or Dye: Alexa Fluor™ 647 Reactive Moiety: sDIBO Alkyne Molecular Weight (g/mol): ∼1900 |
| C20023 | Catalog Number: C20023 Unit Size: 1 mg Label or Dye: Biotin Reactive Moiety: sDIBO Alkyne Molecular Weight (g/mol): ∼1000 |
| C20025 | Catalog Number: C20025 Unit Size: 1 mg Label or Dye: SDP ester Reactive Moiety: SDP ester, sDIBO Alkyne Molecular Weight (g/mol): ∼1050 |
