CytoTune™ EmGFP Sendai Fluorescence Reporter (Invitrogen™)

The CytoTune™ EmGFP Sendai Fluorescence Reporter helps you determine whether the Sendai particles that are used in all CytoTune™-iPS Sendai reprogramming kits can transduce into a given cell type before you advance to one of these kits. This vector expresses the next-generation Aequorea victoria EGFP gene, encoding the emerald green fluorescent protein (EmGFP). The cell will express the EmGFP, which is bright, photostable, and ideal for testing transduction.

The CytoTune™ EmGFP Sendai Fluorescence Reporter enables a better understanding of how both the original CytoTune™-iPS Sendai Reprogramming kits and the newer CytoTune™-iPS 2.0 Sendai Reprogramming Kit transduces various cell types. The Sendai particle enters a cell via its HN envelope protein that recognizes sialic acid and is widely expressed in mammalian cells, allowing Sendai virus to target a wide range of cell types. The Sendai virus vectors do not enter the nucleus, thus avoiding any DNA phase, as the viral genome remains as RNA in the cytoplasm.