Detergent Removal Spin Columns & Plates (Thermo Scientific™)

Detergents and surfactants used to prepare protein and peptide samples can interfere with analysis by ELISA, isoelectric focusing, and mass spectrometry (MS). Removing detergents from peptide samples is especially challenging and critical for MS analysis. Even low concentrations of detergents will contaminate instruments and interfere with column binding, elution, and peptide ionization. Thermo Scientific HiPPR and Pierce detergent removal resins are ideal for rapid removal of common detergents from tryptic digests to improve the results of LC-MS/MS and MALDI-MS analysis.

Key Features

• Optimized—removes >95% of detergent from samples of proteins or peptides
• Fast—sample processing takes less than 15 minutes
• Effective—eliminates detergent-interference in downstream applications like ELISA, isoelectric focusing, and mass spectrometry

Pierce Detergent Removal Resin
Pierce Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 μL to 1 mL samples with minimal sample loss. These microcentrifuge columns come prepacked with various volumes of proprietary resin and are best for processing samples with proteins or peptide concentrations greater than 100 μg/mL.

HiPPR Detergent Removal Spin Column Kit
HiPPR Detergent Removal resin has been optimized for sample concentrations of 1 to 100 μg/mL and is ideal for removing commonly used detergents, including SDS, Triton™ X-100, NP-40 and CHAPS at concentrations of 0.5-1%. The resin is available in pre-filled spin columns, 96-well filter spin plates, and as loose resin for customization.

Available formats:
• Resin slurries—bulk resin supplied with empty 0.8-mL spin columns
• Spin columns—0.1 mL micro-centrifuge columns
• 96-well spin plates—pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification

Order Codes

Code	Description
87777	Catalog Number: 87777 Unit Size: 25 columns Product Line: Pierce™ Product Type: Spin Column Quantity: 25 x 500 μL Columns
87780	Catalog Number: 87780 Unit Size: 10 mL Product Line: Pierce™ Product Type: Detergent Removal Resin Quantity: 10 mL
87776	Catalog Number: 87776 Unit Size: 25 columns Product Line: Pierce™ Product Type: Spin Column Quantity: 25 x 125 μL Columns
87778	Catalog Number: 87778 Unit Size: 5 columns Product Line: Pierce™ Product Type: Spin Column Quantity: 5 x 2 mL Columns
87779	Catalog Number: 87779 Unit Size: 5 columns Product Line: Pierce™ Product Type: Spin Column Quantity: 5 x 4 mL Columns
88304	Catalog Number: 88304 Unit Size: 2 plates Product Line: Pierce™ Product Type: Spin Plate Quantity: 2 x 96-well Plates
88305	Catalog Number: 88305 Unit Size: 5 mL Product Line: HiPPR™ Product Type: Spin Column Quantity: 5 mL
88306	Catalog Number: 88306 Unit Size: 24 columns Product Line: HiPPR™ Product Type: Spin Column Quantity: 24 x 0.1 mL Columns
88307	Catalog Number: 88307 Unit Size: 2 plates Product Line: HiPPR™ Product Type: Spin Plate Quantity: 2 x 96-well Plates