High-Purity Glucoside Detergents (Thermo Scientific™)

Glucoside detergents are a class of non-ionic detergents commonly used in biochemical and biophysical research. These detergents are derived from glucose and possess unique properties that make them suitable for various applications. Glucoside detergents are known for their mildness, biocompatibility, and ability to solubilize and stabilize a wide range of biomolecules, including membrane proteins. Glucoside detergents are also compatible with many downstream applications, such as protein purification, crystallization, and structural studies.

Key Features

Features of glucoside detergents
• Gentle on proteins, minimizing denaturation
• Non-ionic nature—reduces electrostatic interactions
• Low critical micelle concentration (CMC)
• High-purity with low UV absorptivity

Overall, glucoside detergents are versatile and can be used in various experimental techniques, including protein purification, crystallization, and structural biology studies. They are compatible with a wide range of biomolecules and can be employed in both membrane and soluble protein research. With their gentle nature and versatile performance, glucoside detergents have become valuable tools in the field of biochemical research.

Octyl-beta-Glucoside
Octyl-beta-Glucoside (OG) is a low molecular weight, non-ionic detergent with an octyl chain attached to the glucose moiety that has been widely used for membrane protein solubilization. OG is generally more soluble in aqueous solutions and forms clear and stable solutions at higher concentrations. OG typically forms larger micelles compared to OTG. The larger micelles of OG can be advantageous for certain applications, such as protein purification or structural studies, as they provide better solubility and reduced interference during downstream processes.

Properties of OG
Alternative names: octyl-beta-glucopyranoside, octyl-beta-D-glucopyranoside
Molecular weight: 292.37 g
Micelle molecular weight: 8000 g
Critical micelle concentration (CMC): 23 to 25 mM (0.6716 to 0.7300%, w/v)
Aggregation number: 27
Cloud point: >100°C
Optically clear: low absorbance at 280 nm

Octylthio Glucoside
Octylthio Glucoside (OTG) is a low molecular weight, nonionic detergent that is effective for cell lysis and nondenaturing protein solubilization. It is resistant to beta-D-glucoside glucohydrolase degradation because the thioether group present in OTG provides protection against enzymatic hydrolysis. As a result, OTG can remain stable in the presence of beta-D-glucoside glucohydrolase enzymes, making it useful in applications where enzymatic stability is desired. However, it is important to note that the specific stability of OTG can still be influenced by factors such as enzyme concentration, reaction conditions, and the specific enzymes present.

Properties of OTG
Chemical name: n-octyl-β-D-thioglucopyranoside
Molecular weight: 44 g
CMC: 9 mM (0.2772%, w/v)
Cloud point: >100°C
Optically clear: low absorbance at 280 nm

n-Nonyl-β-D-glucoside
n-Nonyl-β-D-glucoside (NG) has a longer alkyl chain (nonyl) compared to other glucosides. The longer alkyl chain in n-Nonyl-β-D-glucoside can confer higher hydrophobicity and potentially stronger detergent properties. This can enhance the solubilization of hydrophobic biomolecules and improve the extraction of membrane proteins. It may also induce different interactions with lipid bilayers, influencing the stability, fluidity, and permeability of the lipid bilayer, potentially impacting the behavior of membrane proteins. In addition, NG forms larger micelles compared to glucosides with shorter alkyl chains.

Properties of NG
CMC: (H2O) ~ 6.5 mM (0.20%)
Aggregation number: ~ 133
Purity: ≥ 99% β+α (by HPLC analysis)
pH: 5-8 (1% solution in water)

Order Codes

Code Description
A65516 Catalog Number: A65516
Unit Size: Each
Description: n-Nonyl-β-D-glucoside (NG)
Quantity: 5 g
A65515 Catalog Number: A65515
Unit Size: Each
Description: n-Nonyl-β-D-glucoside (NG)
Quantity: 1 g
28310 Catalog Number: 28310
Unit Size: Each
Description: Octyl-beta-Glucoside
Quantity: 5 g
28351 Catalog Number: 28351
Unit Size: Each
Description: Octylthioglucoside (OTG)
Quantity: 5 g
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