High Select™ Depletion Spin Columns (Thermo Scientific™)

Analysis of human fluids is often complicated by the presence of high concentrations of serum proteins, including albumin and IgG that can make up more than 70% of total serum protein. Removal of albumin, immunoglobulins, and other abundant proteins is essential for the study of low-abundance proteins. Thermo Scientific High Select HSA/Immunoglobulin and Top 14 Abundant Protein depletion spin columns and resins enable depletion of the most abundant proteins in human serum and plasma for improved detection of low-abundance proteins using mass spectrometry analysis or 1D or 2D gel electrophoresis.

Key Features

• Flexible—resin provided in mini and midi column formats as well as bulk for custom filling options
• Optimized—removes >95% of IgG and >95% of albumin (HSA/Immunoglobulin); removes >95% of IgG and >95% of albumin, plus 12 other abundant proteins (Top 14)
• Fast—process samples in ∼10 minutes
• Economical—cost-effective spin columns are priced for single use
• Reduced carry-over—one-time use prevents protein carryover and experimental variability

High Select HSA/Immunoglobulin Depletion Resin uses highly specific, immobilized anti-HSA and anti-immuoglobulin antibodies (IgG, IgA, IgM, IgD, and IgE) to remove human serum albumin (HSA) and all major subclasses of immunoglobulins from serum and plasma. High Select Top14 Abundant Protein Depletion Resin uses highly specific immobilized antibodies to remove human serum albumin (HSA), albumin, IgG, IgA, IgM, IgD, IgE, kappa and lambda light chains, alpha-1-acidglycoprotein, alpha-1-antitrypsin, alpha-2-macroglobulin, apolipoprotein A1, fibrinogen, haptoglobin, and transferrin from serum, plasma, or spinal fluids. The resins are provided in an economical and convenient spin column format specifically designed for one-step processing and single use or bulk format for custom filling.

Order Codes

Code	Description
A36372	Catalog Number: A36372 Unit Size: 50 mL Description: Top14 Abundant Protein Depletion Resin
A36365	Catalog Number: A36365 Unit Size: 6 columns Description: HSA/Immunoglobulin Depletion Mini Spin Columns
A36366	Catalog Number: A36366 Unit Size: 24 columns Description: HSA/Immunoglobulin Depletion Mini Spin Columns
A36367	Catalog Number: A36367 Unit Size: 10 columns Description: HSA/Immunoglobulin Depletion Midi Spin Columns
A36368	Catalog Number: A36368 Unit Size: 50 mL Description: HSA/Immunoglobulin Depletion Resin
A36371	Catalog Number: A36371 Unit Size: 10 columns Description: Top14 Abundant Protein Depletion Midi Spin Columns
A36369	Catalog Number: A36369 Unit Size: 6 columns Description: Top14 Abundant Protein Depletion Mini Spin Columns
A36370	Catalog Number: A36370 Unit Size: 24 columns Description: Top14 Abundant Protein Depletion Mini Spin Columns