Human MRP3 Vesicles (Gibco™)

Human MRP3 Vesicles are insect derived purified plasma membranes with inserted MRP3 transport protein (Multidrug Resistance-associated Protein 3, ABCC3). Our Human MRP3 Vesicles can be used to evaluate test compounds and drug interactions with MRP3 in in vitro assays. MRP3 is an efflux ATP-binding cassette (ABC) transporter that carries glucuronide conjugates and organic anions, such as methotrexate and leukotriene C4.

Use Human MRP3 Vesicles to:

• Investigate the transporter interactions of your drug candidates
• Assess potential for transporter-mediated drug-drug interactions
• Obtain high quality results with a large signal to noise ratios

Concept Behind ABC Transporter Vesicles
ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate's substrate and drug interaction potential. While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles.

Clear and Reliable Results
Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these 'inside-out' vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter.

Transporter Vesicle Details
Human MRP3 Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing MRP3. MRP3 is is expressed on the basolateral side of small intestinal epithelial cells, where it plays a role in drug and bile acid absorption from the small intestine. It is also present on the basolateral side (vascular side) of hepatocytes, where it plays a role in xenobiotic excretion.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.