Kinase Tracer 178, 25 μM (Thermo Scientific™)

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or “tracer" such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.