Lysozyme (Thermo Scientific™)

Thermo Scientific Lysozyme is an enzyme characterized by the ability to break down the bacterial cell wall to improve protein or nucleic acid extraction efficiency. This product is a lyophilized powder for large-volume applications.

Features of Lysozyme lyophilized powder:

• Bacterial cell wall lytic enzyme that improves inclusion body protein purification
• Compatible with Thermo Scientific Pierce Cell Lysis Reagents
• White, lyophilized powder
• Approx. 20,000 units/mg dry weight
• Must be readily soluble at 5 mg/mL in 0.1M KH2PO4 to give a clear, colorless solution.

Lysozyme is an enzyme used to break down bacterial cell walls to improve protein or nucleic acid extraction efficiency. Lysozymes (muramidases) are a family of enzymes with antimicrobial activity characterized by the ability to damage the cell wall of bacteria. The enzyme acts by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycans and between the N-acetyl-D-glucosamine residues in chitodextrins. Although hen egg white lysozyme is most effective for the lysis of gram-positive bacteria, it also facilitates the lysis of gram-negative bacteria such as Salmonella and Shigella. The lysis of E. coli is especially improved by the addition of both lysozyme and a nucleases such as DNase I.

Description and Properties of Lysozyme:
Lysozyme occurs naturally in plant and animal tissues and in secretions such as tears, saliva and mucus; it is especially abundant in egg whites, which is the primary source for commercial supplies. Hen egg white lysozyme (chick-type or c) was the first enzyme to have its 2-angstrom crystal structure resolved and has been extensively studied. Lysozyme is widely used in bacterial protein extraction. For inclusion body purification, lysozyme is added to digest cell debris and release the inclusion bodies. The enzyme is easily eliminated with other proteins during fusion tag-specific affinity purification of the target recombinant protein in forming the inclusion body.

• Enzymatic function: Catalyzes the hydrolysis of 1,4-β-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycans and between N-acetyl-D-glucosamine residues in chitodextrins
• Molecular weight: 14.388 kDa
• Extinction coefficient: ∼26.4 at 280nm
• Isoelectric point: pH 11.0
• Unit definition: 1 unit is that amount of enzyme needed to catalyze a decrease in absorbance at 450nm of 0.001/min at 25 degrees at pH 6.24 in a 1 cm cuvette due to lysis using a ∼0.25 mg/mL suspension of Micrococcus luteus.

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Lysozyme Solution (50 mg/mL)