Thermo Scientific NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.
Features of NHS-Phosphine:
• Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
• Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
• Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
• Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency
This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine group (PPh2) reacts with azide-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, this NHS-Phosphine reagent facilitates a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.
Related Products
NHS-Phosphine
NHS-Azide
| Code | Description |
|---|---|
| 88900 | Catalog Number: 88900 |
