Pierce™ Horseradish Peroxidase (Thermo Scientific™)

Thermo Scientific Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

Features of Thermo Scientific Pierce Horseradish Peroxidase:

• Purified form—lyophilized salt-free powder; ready to dissolve and use
• High specific activity—typically greater than 300 units/mg (lot-specific value reported)
• Compared to AP—HRP is smaller (40kDa) than alkaline phosphatase (AP; 140kDa) and has higher specific enzyme activity than both AP and beta-galactosidase (b-Gal)
• Many options—numerous substrate solutions and assay techniques are available for HRP

This purified horseradish peroxidase (HRP) is supplied lyophilized as a salt-free powder for reconstitution and use in protein research methods. The main application for HRP in molecular biology and protein research is as a reporter system for immunoassays and other probe-based assay techniques such as ELISA, Western blotting, EMSA and Southern blotting. The enzyme is usually conjugated to specific secondary antibodies or streptavidin, and its activity is detected with a color-forming (or light-generating) substrate.

One unit catalyses the production of 1 mg of purpurogallin from pyrogallol in 20 seconds at 20°C and pH 6.0.