Pierce™ Hydroxylamine-HCl (Thermo Scientific™)

Thermo Scientific Pierce Hydroxylamine-HCl has many uses, which include cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation.

Features of Hydroxylamine-HCl:

• Quench amine-labeling or crosslinking reactions (e.g., with NHS esters)
• Expose protected sulfhydryl groups on SATA-modified molecules
• Cleave carbonyl-containing crosslinkers such as EGS and Sulfo-EGS

Hydroxylamine·HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine·HCl.