Pierce™ Iminobiotin Agarose (Thermo Scientific™)

Thermo Scientific Pierce Iminobiotin Agarose provides for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components.

Features of Iminobiotin Agarose:

• Enable purification or removal of avidin or streptavidin samples
• Crosslinked beaded agarose onto which iminobiotin is covalently immobilized
• Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)

Pierce Iminobiotin Agarose is beaded agarose support onto which iminobiotin moieties have covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin is needed to capture avidin or streptavidin components from a sample. This iminobiotin resin addresses this special situation.

Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Because no denaturing agents such as 8M guanidine hydrochloride or 4M urea are required for elution, the avidin conjugate has a better chance of maintaining its activity after purification. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.