Pierce™ Protein A/G IgG Binding Buffer (Thermo Scientific™)

Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Protein A/G IgG Binding Buffer is a phosphate-based buffer containing EDTA, formulated for antibody purification using either Protein A or Protein G.

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Shared features of all Thermo Scientific Pierce IgG Binding and Elution Buffers:

• Ready to use—carefully formulated and prefiltered 1X solutions
• Three binding buffers—optimized for Protein A, Protein G or Protein A/G
• Two elution buffer options—acidic (pH 2.8) or high salt (near-neutral)
• Versatile—elution buffers are appropriate for antibody-antigen affinity purification

Products available for each antibody purification method:
• Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
• Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
• Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
• IgG Elution Buffer: pH 2.8; amine-based
• Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
• Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

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Pierce™ IgG Elution Buffer