PNGase F Glycan Cleavage Kit (Gibco™)

The PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins. The kit includes the PNGase F enzyme, which cleaves N-glycan chains at the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.

The PNGase F Glycan Cleavage Kit features include:
• ≥95% purity
• Recombinant PNGase F enzyme with no endoglycosidase F1, F2, or F3
• Enzyme leaves N-glycan core oligosaccharides intact and suitable for downstream analysis
• Suitable for use under native or denaturing conditions

The components included in the PNGase F Glycan Cleavage Kit are: 33,000 units of PNGase F (500,000 units/mL) and 1 mL PNGase F 10X buffer.