POROS™ GoPure™ Benzyl Ultra Pre-packed Column, 0.5 x 5 cm, 1 mL (Thermo Scientific™)

POROS GoPure 0.5 cmD pre-packed columns are intended for the screening of POROS ion exchange and hydrophobic interaction chromatography (HIC) resins or for small-scale purification. These pre-packed columns are ready to connect and have the following features:

• Dimensions of 0.5 cmD x 5 cmL, with a nominal bed volume of 1 ml, providing a uniform screening platform
• Direct connection to standard chromatography systems
• Packed with POROS high-performance resins that offer high dynamic binding capacity and high resolution over a wide range of flow rates

The POROS Benzyl Ultra HIC Resin in this GoPure column has an aromatic hydrophobic benzyl ligand, well suited for hydrophobic interaction chromatography (HIC) for aggregate removal from monoclonal antibody monomer in flow-through mode at low salt concentrations.

• Convenient—ready-to-run columns simplify and speed POROS resin evaluations
• Conserves sample—the 1 mL nominal bed volume minimizes sample requirement for screening multiple resins
• Simplified scalability—the consistency and range of the POROS GoPure family of pre-packed columns simplifies process scale-up and process modeling

POROS GoPure 0.5 cmD pre-packed columns have been developed to meet the needs of the process development scientist requiring a convenient and uniform POROS resin-screening platform. The 1 mL bed volume reduces sample requirements, and the recommended > 3 min residence time (0.3 mL/min) on this 5-cm-length column equates to 400 cm/hr on a 20-cm bed height. Detailed specifications may be found in the POROS GoPure 0.5 cmD Operating Instructions below.

Other POROS hydrophobic interaction and ion exchange resins are also available in this column format. All POROS High Performance Chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.

For scaleup, POROS GoPure pre-packed columns are available in a range of diameters and in multiple bed heights that equate to a column volume range of 5 mL to 22 mL.