The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pTK-Green Renilla Luc vector:
• Weak (TK) constitutive promoter for co-transfection and normalization
• Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
More Product Data
• Luciferase assays in hard-to-transfect Jurkat cells
Related Products
pMCS-Green Renilla Luc Vector for Luciferase Assays
pCMV-Green Renilla Luc Vector for Luciferase Assays
| Code | Description |
|---|---|
| 16154 | Catalog Number: 16154 |
