RadPrime DNA Labeling System (Invitrogen™)

The RadPrime DNA Labeling System is ideal for rapid preparation (under 10 min) of high-specific activity 32 P-labeled probes for the detection of DNA and RNA (1). The RadPrime DNA Labeling System:

• Produces probes that can detect nucleic acids on northern or Southern blots, plaque lifts, colony hybridizations, and in situ hybridization
• Yields >10⁹ cpm/μg control DNA using [³²P]-dCTP
• Labels 25 ng of DNA in one reaction

Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verified using control DNA in a RadPrime labeling reaction.