Rapid ELISA Mouse mAb Isotyping Kit (Thermo Scientific™)

The Thermo Scientific Pierce Rapid ELISA Mouse mAb Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using a microplate method that enables multiple samples to be tested in about one hour.

Features of the Rapid ELISA Mouse mAb Isotyping Kit:

• Fast—determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits
• Convenient—eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)
• Specific—characterize antibodies for six different subclasses and two different light-chain types
• Sensitive—accurate characterization with samples containing at least 3 ng/mL of test antibody
• Flexible—kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies
• Cost effective—cost per assay is less than with single-use assay strips or cassettes
• No special equipment needed—assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)
• Complete and easy to use—includes precoated plates, detecting antibody, buffers, and TMB substrate and stop solutions

This fast assay uses ELISA strip-plates that are pre-coated in different wells with anti-mouse heavy-chain capture antibodies (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This approach eliminates the need to purify and immobilize an antigen to determine immunoglobulin isotype (antibody subclass and light chain identity). A mouse monoclonal antibody sample applied to the wells can be isotyped within one hour. Results are evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450nm.

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