ReadyProbes™ Tissue Autofluorescence Quenching Kit (Invitrogen™)

The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes unwanted autofluorescence in FFPE and frozen tissues due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin. If the target signal is low enough, then this background signal can interfere with the interpretation of immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence(IF), and in situ hybridization (ISH) assays. The ReadyProbes Tissue Autofluorescence Quenching Kit minimizes most kinds of autofluorescence, with the exception of autofluorescence from lipofuschin granules.

The ReadyProbes Tissue Autofluorescence Quenching Kit contains three components that are combined just before use. Combine components A, B, and C in a 1:1:1 ratio. Mix components A and B together before adding component C. The resulting solution is then applied to the tissue for 2–5 minutes at room temperature. This is done after the blocking, primary antibody, and secondary antibody steps, but before application of any counterstains like nuclear stains.