SiteClick™ sDIBO Alkyne Kits for Antibody Labeling (Invitrogen™)

Generate site-specific labeled antibody conjugates with SiteClick sDIBO Alkynes for Antibody Labeling. Our SiteClick sDIBO Antibody Labeling kits are optimized to be used in conjunction with the SiteClick Azido Modification kits (S20026, S10900, or S10901 sold separately) and contain sufficient reagents to perform a conjugation reaction of a SiteClick sDIBO Alkyne label using 100 μg, 1 mg, or 5 mg of azido-modified IgG antibody. The SiteClick sDIBO Alkynes for Antibody Labeling employs copper-free click chemistry to covalently link a dibenzocyclooctyne (sDIBO) labeled Alexa Fluor dye, pHrodo dye, or biotin to an azido modified antibody. The label, when attached to the antibody, is located far away from its antigen binding domain.

Key Features

Create your own covalently labeled antibody conjugates using the SiteClick sDIBO Alkyne labels, which are intended for use with azido-modified antibodies. SiteClick sDIBO Alkyne Antibody Labeling Kits include the sDIBO Alkyne and the antibody concentrator, enabling copper-free covalent attachment of Alexa Fluor and pHrodo dyes, biotin, or molecules of your choice. When used with the SiteClick Antibody Azido Modification Kit (available separately as Cat. No. S20026, S10900, S10901), labeling occurs on the heavy chain N-linked glycans, far away from the antigen-binding domain, enabling excellent reproducibility between separate labeling reactions and multiple antibody species and subtypes. The SiteClick sDIBO alkyne kits for antibody labeling are optimized for conjugation reactions, covalently linking 100 μg, 1 mg, or 5 mg of azide-modified IgG antibody.

The SiteClick Antibody Azido Modification Kit (Cat. No. S20026, S10900, S10901) is recommended for the attachment of an azide moiety to the heavy chains of an unlabeled IgG antibody. Other engineered antibodies containing azido moieties are also suitable.

When used in conjunction with the SiteClick Antibody Azido Modification Kit, the SiteClick sDIBO alkyne kits for antibody labeling give you the option of trying different fluorescent labels for your antibody, attaching other molecules via streptavidin, or attaching your own molecule via amine-reactive moieties, depending on your assay. Our sDIBO alkynes are improved versions of DIBO cyclooctynes, yielding conjugates that are less 'sticky' and emit lower signal background in biological samples.

Order Codes

Code	Description
S10909	Catalog Number: S10909 Unit Size: for 5 mg of Ab Label or Dye: Alexa Fluor 488 Labeling Scale: 5 mg
C20027	Catalog Number: C20027 Unit Size: 1 each Label or Dye: Alexa Fluor 488 Labeling Scale: 100-250 μg
C20029	Catalog Number: C20029 Unit Size: 1 each Label or Dye: Alexa Fluor 647 Labeling Scale: 100-250 μg
C20031	Catalog Number: C20031 Unit Size: 1 each Label or Dye: Primary Amine Labeling Scale: 100-250 μg
C20034	Catalog Number: C20034 Unit Size: 1 kit Label or Dye: pHrodo Red Labeling Scale: 100-250 μg
S10902	Catalog Number: S10902 Unit Size: for 1 mg of Ab Label or Dye: Biotin Labeling Scale: 1 mg
S10903	Catalog Number: S10903 Unit Size: for 1 mg of Ab Label or Dye: pHrodo Red Labeling Scale: 1 mg
S10904	Catalog Number: S10904 Unit Size: for 1 mg of Ab Label or Dye: Alexa Fluor 488 Labeling Scale: 1 mg
S10906	Catalog Number: S10906 Unit Size: for 1 mg of Ab Label or Dye: Alexa Fluor 647 Labeling Scale: 1 mg
S10907	Catalog Number: S10907 Unit Size: for 5 mg of Ab Label or Dye: Biotin Labeling Scale: 5 mg
S10908	Catalog Number: S10908 Unit Size: for 5 mg of Ab Label or Dye: pHrodo Red Labeling Scale: 5 mg
S10911	Catalog Number: S10911 Unit Size: for 5 mg of Ab Label or Dye: Alexa Fluor 647 Labeling Scale: 5 mg
S10914	Catalog Number: S10914 Unit Size: For up to 250 µg of Ab Label or Dye: pHrodo Deep Red Labeling Scale: 100-250 μg
S10915	Catalog Number: S10915 Unit Size: For up to 1 mg of Ab Label or Dye: pHrodo Deep Red Labeling Scale: 1 mg
C20028	Catalog Number: C20028 Unit Size: 1 each Label or Dye: Alexa Fluor 555 Labeling Scale: 100-250 μg
C20030	Catalog Number: C20030 Unit Size: 1 each Label or Dye: Biotin Labeling Scale: 100-250 μg