Slide-A-Lyzer™ Dialysis Cassettes, 20K MWCO (Thermo Scientific™)

Slide-A-Lyzer Dialysis Cassettes (20K MWCO) facilitate the simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules larger than 20,000 Daltons. They are available in four sizes to accommodate sample volumes between 0.5 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing faster buffer exchange, and more complete sample recovery.

Key Features

Slide-A-Lyzer Dialysis Cassettes (20K MWCO) facilitate the simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules larger than 20,000 Daltons. They are available in four sizes to accommodate sample volumes between 0.5 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing faster buffer exchange and more complete sample recovery. Slide-A-Lyzer cassettes can be used for a wide range of applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips, or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

Features of Slide-A-Lyzer Dialysis Cassettes:
Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—purple cassette frame specifies cassettes with the 20,000 MW pore size membrane

Membrane specifications:
Composition: regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2 mil (22.5 to 30 μm)
Hydration time required before use: 2 minutes
Glycerol content: none
Sulfur content: 0.04%
Heavy metals content: trace

Volume ranges:
• 0.5 mL = 0.1 to 0.5 mL
• 3 mL = 0.5 to 3 mL
• 12 mL = 3 to 12 mL
• 30 mL = 12 to 30 mL

Order Codes

Code Description
66030 Catalog Number: 66030
Unit Size: 6 cassettes
Sample Volume (Metric): 30 mL
66005 Catalog Number: 66005
Unit Size: 10 cassettes
Sample Volume (Metric): 0.5 mL
66003 Catalog Number: 66003
Unit Size: 10 cassettes
Sample Volume (Metric): 3 mL
66012 Catalog Number: 66012
Unit Size: 8 cassettes
Sample Volume (Metric): 12 mL
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