Streptavidin, Alexa Fluor™ 647 Conjugate (Invitrogen™)

Alexa Fluor™ 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 647 Streptavidin Conjugates:
Alexa Fluor™ 647 streptavidin conjugate has Ex/Em maxima of ∼ (650/668)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

Consult user Manual for solubility instructions.

Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor™ Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

Order Codes

Code Description
S32357 Catalog Number: S32357
S21374 Catalog Number: S21374
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